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Laminin peptide‐conjugated chitosan membrane: Application for keratinocyte delivery in wounded skin by Shigehiro Ikemoto; Mayumi Mochizuki; Masanori Yamada; Akira Takeda; Eiju Uchinuma; Shohei Yamashina; Motoyoshi Nomizu; Yuichi Kadoya is a Engineering article available to read on EtoBox.

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## Abstract Tissue engineering requires the delivery and survival of cells to organ sites needing repair. Previously, we showed that an active laminin peptide (AG73: RKR‐LQVQLSIRT)‐conjugated chitosan membrane promoted cell adhesion and spreading __in vitro__. Here, we seeded human keratinocytes onto AG73‐chitosan membranes and found that nearly 80% of the cells were attached to the membranes within 2 h. The membranes carrying the keratinocytes were inverted and placed onto exposed muscle fascia on the backs of nude mice. After 3 days, the keratinocytes had migrated from the membrane and established a stratified epidermis‐like structure on the fascia. Cells recognize the AG73 through transmembrane proteoglycan syndecans, which recognition system has not previously been tested in tissue engineering applications. We suggest that the AG73‐chitosan membrane is useful as a therapeutic formulation and is applicable as a cell delivery system such as delivering keratinocytes to a wound bed. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

Who reads Laminin peptide‐conjugated chitosan membrane: Application for keratinocyte delivery in wounded skin?

Author

Shigehiro Ikemoto; Mayumi Mochizuki; Masanori Yamada; Akira Takeda; Eiju Uchinuma; Shohei Yamashina; Motoyoshi Nomizu; Yuichi Kadoya

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2006

Language

EN

Field

Engineering (Physical Sciences)