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Can I read Electronegative LDL Promotes Inflammation and Triglyceride Accumulation in Macrophages on EtoBox?
Electronegative LDL Promotes Inflammation and Triglyceride Accumulation in Macrophages by Puig, NÃoria; Montolio, Lara; Camps-Renom, Pol; Navarra, Laia; Jiménez-Altayó, Francesc; Jiménez-Xarrié, Elena; Sánchez-Quesada, Jose Luis; Benitez, Sonia is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Electronegative LDL Promotes Inflammation and Triglyceride Accumulation in Macrophages about?
Electronegative low-density lipoprotein (LDL) (LDL(−)), a modified LDL that is present in blood and exerts atherogenic effects on endothelial cells and monocytes. This study aimed to determine the action of LDL(−) on monocytes differentiated into macrophages. LDL(−) and in vitro-modified LDLs (oxidized, aggregated, and acetylated) were added to macrophages derived from THP1 monocytes over-expressing CD14 (THP1-CD14). Then, cytokine release, cell differentiation, lipid accumulation, and gene expression were measured by ELISA, flow cytometry, thin-layer chromatography, and real-time PCR, respectively. LDL(−) induced more cytokine release in THP1-CD14 macrophages than other modified LDLs. LDL(−) also promoted morphological changes ascribed to differentiated macrophages. The addition of high-density lipoprotein (HDL) and anti-TLR4 counteracted these effects. LDL(−) was highly internalized by macrophages, and it was the major inductor of intracellular lipid accumulation in triglyceride-enriched lipid droplets. In contrast to inflammation, the addition of anti-TLR4 had no effect on lipid accumulation, thus suggesting an uptake pathway alternative to TLR4. In this regard, LDL(−) upregulat
Who reads Electronegative LDL Promotes Inflammation and Triglyceride Accumulation in Macrophages?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Puig, NÃoria; Montolio, Lara; Camps-Renom, Pol; Navarra, Laia; Jiménez-Altayó, Francesc; Jiménez-Xarrié, Elena; Sánchez-Quesada, Jose Luis; Benitez, Sonia
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI) (ISSN 2073-4409)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)