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De Novo Mutation Rate Variation and Its Determinants in Chlamydomonas by Eugenio López-Cortegano; Rory J Craig; Jobran Chebib; Toby Samuels; Andrew D Morgan; Susanne A Kraemer; Katharina B Böndel; Rob W Ness; Nick Colegrave; Peter D Keightley is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract De novo mutations are central for evolution, since they provide the raw material for natural selection by regenerating genetic variation. However, studying de novo mutations is challenging and is generally restricted to model species, so we have a limited understanding of the evolution of the mutation rate and spectrum between closely related species. Here, we present a mutation accumulation (MA) experiment to study de novo mutation in the unicellular green alga Chlamydomonas incerta and perform comparative analyses with its closest known relative, Chlamydomonas reinhardtii. Using whole-genome sequencing data, we estimate that the median single nucleotide mutation (SNM) rate in C. incerta is μ = 7.6 × 10−10, and is highly variable between MA lines, ranging from μ = 0.35 × 10−10 to μ = 131.7 × 10−10. The SNM rate is strongly positively correlated with the mutation rate for insertions and deletions between lines (r > 0.97). We infer that the genomic factors associated with variation in the mutation rate are similar to those in C. reinhardtii, allowing for cross-prediction between species. Among these genomic factors, sequence context and complexity are more important than

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Author

Eugenio López-Cortegano; Rory J Craig; Jobran Chebib; Toby Samuels; Andrew D Morgan; Susanne A Kraemer; Katharina B Böndel; Rob W Ness; Nick Colegrave; Peter D Keightley

Publisher

Oxford University Press (OUP)

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)