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Can I read A Simplified Monobuffer Multidimensional Chromatography for High-throughput Proteome Fractionation on EtoBox?
A Simplified Monobuffer Multidimensional Chromatography for High-throughput Proteome Fractionation by L. Guerrier; L. Lomas; E. Boschetti is a Chemistry article available to read on EtoBox.
What is A Simplified Monobuffer Multidimensional Chromatography for High-throughput Proteome Fractionation about?
The complexity of the human serum proteome is attributed to both a large dynamic range of protein abundance, as much as 10 orders of magnitude, and a disproportionate few dozens of proteins representing as much as 99% of the total protein content. These characteristics make it beneficial to use a pre-fractionation step prior to any high-resolution analysis, such as mass spectrometry. The present method describes a unimodal multidimensional chromatography concept to rapidly achieve an effective fractionation of human serum that is directly amenable with surface-enhanced laser desorption/ionization (SELDI)-based mass spectrometry. This method is based on the use of a column composed of a superimposed sequence of sorbents. The assembly is first equilibrated with a single binding buffer and then loaded with the whole crude sample. As the sample crosses the different adsorbent layers proteins within are sequentially trapped according to the complementary properties vis-a-vis of the sorbent. Once the loading and capturing is achieved, the sequence of columns is disassembled and each column, containing different complement of proteins is eluted separately in a single step and under optima
Who reads A Simplified Monobuffer Multidimensional Chromatography for High-throughput Proteome Fractionation?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- L. Guerrier; L. Lomas; E. Boschetti
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2005
- Language
- EN
- Field
- Chemistry (Physical Sciences)