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Can I read Whole Cell Matrix‐assisted Laser Desorption/ionization Time‐of‐flight Mass Spectrometry and in Situ Structure Analysis of Streptocidins, a Family of Tyrocidine‐like Cyclic Peptides on EtoBox?
Whole Cell Matrix‐assisted Laser Desorption/ionization Time‐of‐flight Mass Spectrometry and in Situ Structure Analysis of Streptocidins, a Family of Tyrocidine‐like Cyclic Peptides by Gabriele Hitzeroth; Joachim Vater; Peter Franke; Klaus Gebhardt; Hans‐Peter Fiedler is a Chemistry article available to read on EtoBox.
What is Whole Cell Matrix‐assisted Laser Desorption/ionization Time‐of‐flight Mass Spectrometry and in Situ Structure Analysis of Streptocidins, a Family of Tyrocidine‐like Cyclic Peptides about?
## Abstract Streptocidins, a family of tyrocidine‐like cyclic decapeptides, are an ideal demonstration object for the detection and __in situ__ structure analysis of natural compounds directly in microbial cells using whole cell matrix‐assisted laser desorption/ionization time‐of‐flight‐mass spectrometry (MALDI‐TOFMS), an emerging technique that can be used for rapid sensitive metabolic profiling of microorganisms. Five main members of the streptocidin family (A–E) were detected in __Brevibacillus__ cells picked from agar plates and identified by __in situ__ structure analysis with post‐source decay MALDI‐TOFMS. This efficient modern method allows the precise detection of metabolites within minutes without the need to isolate and purify the target compounds. The generated mass spectra are of similar quality to those obtained for the purified peptides. In addition, surface extracts were prepared by treating __Brevibacillus__ cells with 70% acetonitrile in the presence of 0.1% trifluoroacetic acid and fractionated by high‐resolution reversed‐phase high‐performance liquid chromatography (HPLC). In this way ten minor streptocidins were detected demonstrating the full biosynthetic varie
Who reads Whole Cell Matrix‐assisted Laser Desorption/ionization Time‐of‐flight Mass Spectrometry and in Situ Structure Analysis of Streptocidins, a Family of Tyrocidine‐like Cyclic Peptides?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Gabriele Hitzeroth; Joachim Vater; Peter Franke; Klaus Gebhardt; Hans‐Peter Fiedler
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2005
- Language
- EN
- Field
- Chemistry (Physical Sciences)