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Engineered Cardiac Tissue Microsphere Production Through Direct Differentiation of Hydrogel-encapsulated Human Pluripotent Stem Cells by Ferdous B. Finklea; Yuan Tian; Petra Kerscher; Wen J. Seeto; Morgan E. Ellis; Elizabeth A. Lipke is a Engineering article available to read on EtoBox.
What is Engineered Cardiac Tissue Microsphere Production Through Direct Differentiation of Hydrogel-encapsulated Human Pluripotent Stem Cells about?
Engineered cardiac tissues that can be directly produced from human induced pluripotent stem cells (hiPSCs) in scalable, suspension culture systems are needed to meet the demands of cardiac regenerative medicine. Here, we demonstrate successful production of functional cardiac tissue microspheres through direct differentiation of hydrogel encapsulated hiPSCs. To form the microspheres, hiPSCs were suspended within the photocrosslinkable biomaterial, PEG-fibrinogen (25 million cells/mL), and encapsulated at a rate of 420,000 cells/minute using a custom microfluidic system. Even at this high cell density and rapid production rate, high intra-batch and batch-to-batch reproducibility was achieved. Following microsphere formation, hiPSCs maintained high cell viability and continued to grow within and beyond the original PEG-fibrinogen matrix. These initially soft microspheres ( 75% cardiomyocytes (CMs). CMs responded appropriately to pharmacological stimuli and exhibited 1:1 capture up to 6.0 Hz when electrically paced. Over time, cells formed cell-cell junctions and aligned myofibril fibers; engineered cardiac microspheres were maintained in culture over 3 years. The capability to rapid
Who reads Engineered Cardiac Tissue Microsphere Production Through Direct Differentiation of Hydrogel-encapsulated Human Pluripotent Stem Cells?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Ferdous B. Finklea; Yuan Tian; Petra Kerscher; Wen J. Seeto; Morgan E. Ellis; Elizabeth A. Lipke
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Engineering (Physical Sciences)