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Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay by Xiong Ding; Kun Yin; Ziyue Li; Maroun M. Sfeir; Changchun Liu is a Engineering article available to read on EtoBox.
What is Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay about?
Quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical samples is crucial for early diagnosis and timely medical treatment of coronavirus disease 2019. Here, we describe a digital warm-start CRISPR (dWS-CRISPR) assay for sensitive quantitative detection of SARS-CoV-2 in clinical samples. The dWS-CRISPR assay is initiated at above 50 °C and overcomes undesired premature target amplification at room temperature, enabling accurate and reliable digital quantification of SARS-CoV-2. By targeting SARS-CoV-2's nucleoprotein gene, the dWS-CRISPR assay is able to detect down to 5 copies/μl SARS-CoV-2 RNA in the chip. It is clinically validated by quantitatively determining 32 clinical swab samples and three clinical saliva samples. Moreover, it has been demonstrated to directly detect SARS-CoV-2 in heat-treated saliva samples without RNA extraction. Thus, the dWS-CRISPR method, as a sensitive and reliable CRISPR assay, facilitates accurate SARS-CoV-2 detection toward digitized quantification.
Who reads Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Xiong Ding; Kun Yin; Ziyue Li; Maroun M. Sfeir; Changchun Liu
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Engineering (Physical Sciences)