Can I read Lentiviral Vector Production, Titration, and Transduction of Primary Neurons on EtoBox?

Lentiviral Vector Production, Titration, and Transduction of Primary Neurons by Baojin Ding; Daniel L. Kilpatrick is a book available to read on EtoBox.

What is Lentiviral Vector Production, Titration, and Transduction of Primary Neurons about?

Lentiviral vectors have become very useful tools for transgene delivery. Based on their ability to transduce both dividing and nondividing cells and to produce long-term transgene expression, lentiviruses have found numerous applications in the biomedical sciences, including developmental neuroscience. This protocol describes how to prepare lentiviral vectors by calcium phosphate transfection and to concentrate viral particles by ultracentrifugation. Functional vector titers can then be determined by methods such as fl uorescence-activated cell sorting or immunostaining. Effective titers in the range of 10 8 -10 9 infectious units/ml can be routinely obtained using these protocols. Finally, we describe the infection of primary neuronal cultures with lentiviral vectors resulting in 85-90 % cell transduction using appropriate multiplicities of infection.

Author

Baojin Ding; Daniel L. Kilpatrick

Publisher

Humana Press : Imprint : Humana Press

Published

2013

Language

EN

ISBN

9781627034449

Subjects

Medical, Science, Biology