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Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis by Shi, Wenqi (author);Li, Jie (author);Chen, Yanfang (author);Liu, Xiaohang (author);Chen, Yefu (author);Guo, Xuewu (author);Xiao, Dongguang (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis about?

Ethyl acetate can be synthesized from acetyl-CoA and ethanol via a reaction by alcohol acetyltransferases (AATase) in yeast. In order to increase the yield of acetyl-CoA, different terminators were used to optimize the expressions of acetyl-CoA synthetase (ACS1/2) and aldehyde dehydrogenase (ALD6) to increase the contents of acetyl-CoA in Saccharomyces cerevisiae. ATF1 coding AATase was coexpressed in expression cassettes of ACS1/ACS2 and ALD6 to promote the carbon flux toward ethyl acetate from acetyl-CoA. Further to improve ethyl acetate production, four heterologous AATase including HuvEAT1 (Hanseniaspora uvarum), KamEAT1 (Kluyveromyces marxianus), VAAT (wild strawberry), and AeAT9 (kiwifruit) were introduced. Subsequently mitochondrial transport and utilization of pyruvate and acetyl-CoA were impeded to increase the ethyl acetate accumulation in cytoplasm. Under the optimal fermentation conditions, the engineered strain of PGAeΔPOR2 produced 1.69 g/L ethyl acetate, which was the highest value reported to date by metabolic engineering methods.

Who reads Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis?

Author

Shi, Wenqi (author);Li, Jie (author);Chen, Yanfang (author);Liu, Xiaohang (author);Chen, Yefu (author);Guo, Xuewu (author);Xiao, Dongguang (author)

Publisher

American Chemical Society (ACS)

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)