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METHODS IN MOLECULAR BIOLOGY VOLUME 205 E.COLI GENE EXPRESSION PROTOCOLS by François Baneyx, Mirna Mujacic (auth.), Peter E. Vaillancourt (eds.) is a nonfiction available to read on EtoBox.

What is METHODS IN MOLECULAR BIOLOGY VOLUME 205 E.COLI GENE EXPRESSION PROTOCOLS about?

Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection o

Who reads METHODS IN MOLECULAR BIOLOGY VOLUME 205 E.COLI GENE EXPRESSION PROTOCOLS?

Author

François Baneyx, Mirna Mujacic (auth.), Peter E. Vaillancourt (eds.)

Publisher

Humana Press : Imprint : Humana

Published

2003

Language

EN

ISBN

9781592593019

Category

nonfiction

Subjects

Science, Chemistry, Medical