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Comparison between Wako-WB003 and Fungitec G tests for detection of (1→3)-β-D-glucan in systemic mycosis by Mohammad Ashraf Hossain; Takashige Miyazaki; Kotaro Mitsutake; Hiroshi Kakeya; Yoshihiro Yamamoto; Katsunori Yanagihara; Sumio Kawamura; Takakazu Otsubo; Yoichi Hirakata; Takayoshi Tashiro; Shigeru Kohno is a Medicine article available to read on EtoBox.
What is Comparison between Wako-WB003 and Fungitec G tests for detection of (1→3)-β-D-glucan in systemic mycosis about?
The limulus factor G reacts with (1-->3)-beta-D-glucan, a major structural component of fungal cell walls. The Fungitec G test is a colorimetric assay that measures the concentration of (1-->3)-beta-D-glucan and is used as a serodiagnostic test for deep mycosis. Wako-WB003 is another assay for (1-->3)-beta-D-glucan that determines the change in turbidity of the gelatin reaction of limulus factor G with (1-->3)-beta-D-glucan. In five rabbits inoculated intravenously with 1 x 10(7) CFU of Candida albicans, the concentration of (1-->3)-beta-D-glucan measured by the fungitec G test increased gradually reaching a peak of 660.9 +/- 427.9 pg/ml (mean +/- SD) 4 days after inoculation, but to 42.225 +/- 41.275 ng/ml on day 6 in the Wako-WB003 test. In one rabbit challenged intravenously with 5 x 10(6) CFU of C. albicans, (1-->3)-beta-D-glucan increased to 101.5 pg/ml on day 4 on the fungitec G test, whereas the level remained below the detection limit of the Wako-WB003 test throughout the course of the disease. We also detected high concentrations of (1-->3)-beta-D-glucan in 11 patients with candidemia, 4 with suspected candidemia, 1 with invasive pulmonary aspergillosis, and 12 patients wi
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- Author
- Mohammad Ashraf Hossain; Takashige Miyazaki; Kotaro Mitsutake; Hiroshi Kakeya; Yoshihiro Yamamoto; Katsunori Yanagihara; Sumio Kawamura; Takakazu Otsubo; Yoichi Hirakata; Takayoshi Tashiro; Shigeru Kohno
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0887-8013)
- Published
- 1997
- Language
- EN
- Field
- Medicine (Health Sciences)