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Metabolite Changes in the Cerebral Cortex of Treated and Untreated Infant Hydrocephalic Rats Studied Using In Vitro ^31^P‐NMR Spectroscopy by N. G. Harris; H. D. Plant; R. W. Briggs; H. C. Jones is a Neuroscience article available to read on EtoBox.

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Abstract: The effect of hydrocephalus on cerebral energy metabolites and on intermediates of membrane phospholipid metabolism has been studied in H‐Tx rats with inherited infantile hydrocephalus. Hydrocephalic rats and rats with shunts placed at 4–5 days or at 10 days after birth were subjected to magnetic resonance imaging in vivo before 21 days of age to determine the dimensions of the ventricles and cortex. At 21 days, the brains from the three groups of rats, together with age‐matched control littermates, were frozen in situ, and chloroform/methanol extracts of cerebral cortex were prepared for high‐resolution ^31^P‐NMR spectroscopy. Hydrocephalus resulted in modest decreases in most metabolites quantified. Levels of phosphocreatine, ATP, and diphosphodiesters plus NAD were significantly reduced by 23–32%, and inorganic phosphate content was reduced but not significantly. Levels of the membrane phospholipid intermediates phosphorylethanolamine, glycerophosphorylethanolamine, and glycerophosphorylcholine were also significantly reduced by 30–33%, indicating changes in membrane metabolism. These general decreases are consistent with a loss of cell contents, possibly due to change

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Author

N. G. Harris; H. D. Plant; R. W. Briggs; H. C. Jones

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1996

Language

EN

Field

Neuroscience (Life Sciences)