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Reverse-phase phosphoproteome analysis (RPMA) of signaling pathways induced by HTLV-1 infection by Taissia G Popova; Aarthi Narayanan; Lance Liotta; Emanuel F Petricoin III; Charles Bailey; Kylene Kehn-Hall; Fatah Kashanchi is a Immunology and Microbiology article available to read on EtoBox.

What is Reverse-phase phosphoproteome analysis (RPMA) of signaling pathways induced by HTLV-1 infection about?

Phosphorylation plays a key role in regulating many signaling pathways. Cell fate decisions in response to extracellular agents, including pathogenic invaders, are commonly mediated by phosphorylation-regulated signaling cascades that transduce signals into stimulusspecific actions, such as changes in gene expression pattern. Here we utilized a novel approach where we infected cells with HTLV wild type and mutant clones and analyzed the phosphorylation status of the cells. For phosphoproteomic analysis the cell lysates were printed onto nitrocellulose membrane slides. Each slide was then probed with one of 360 different antibodies specific against phosphorylated or total forms of signaling proteins. The antibodies were selected to monitor the molecular networks involved in host responses most likely affected by virus exposure, namely survival, apoptosis, inflammation, growth, differentiation, and immune response. This RPMA technique was extensively validated in our previous studies with regard to specificity of antibodies, sensitivity, and accuracy of phosphoprotein detection in cell lysates. Changes in NFkB, SAP/ JNK, ERK and AKT and against phosphorylated form of p-38 and PTEN wi

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Author

Taissia G Popova; Aarthi Narayanan; Lance Liotta; Emanuel F Petricoin III; Charles Bailey; Kylene Kehn-Hall; Fatah Kashanchi

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, c2004-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1742-4690)

Published

2011

Language

EN

Field

Immunology and Microbiology (Life Sciences)