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Photoactivation of pheophorbide a induces a mitochondrial-mediated apoptosis in Jurkat leukaemia cells by Won-Young Lee; Dae-Seog Lim; Si-Hwan Ko; Young-Jae Park; Kang-Sun Ryu; Mi-Young Ahn; Yong-Rok Kim; Dai Woon Lee; Chang-Whan Cho is a Medicine article available to read on EtoBox.
What is Photoactivation of pheophorbide a induces a mitochondrial-mediated apoptosis in Jurkat leukaemia cells about?
The mechanism of cell death by pheophorbide a (Pba) which has been established to be a potential photosensitizer was examined in experimental photodynamic therapy (PDT) on Jurkat cells, a human lymphoid tumor cell line. In 30-60 min after irradiation, Pba treated cells exhibited apoptotic features including membrane blebbing and DNA fragmentation. Pba/PDT caused a rapid release of cytochrome c from mitochondria into the cytosol. Sequentially, activation of caspase-3 and the cleavage of poly ADP-ribose polymerase (PARP) were followed. Meanwhile, no evidence of activation of caspase-8 was indicated in the cells. In experiments with caspase inhibitors, it was found that caspase-3 alone was sufficient initiator for the Pba-induced apoptosis of the cells. Pba specific emission spectra were confirmed in the mitochondrial fraction and the light irradiation caused a rapid change in its membrane potential. Thus, mitochondria were entailed as the crucial targets for Pba as well as a responsible component for the cytochrome c release to initiate apoptotic pathways. Taken together, it was concluded that the mode of Jurkat cell death by Pba/PDT is an apoptosis, which is initiated by mitochondri
Who reads Photoactivation of pheophorbide a induces a mitochondrial-mediated apoptosis in Jurkat leukaemia cells?
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- Author
- Won-Young Lee; Dae-Seog Lim; Si-Hwan Ko; Young-Jae Park; Kang-Sun Ryu; Mi-Young Ahn; Yong-Rok Kim; Dai Woon Lee; Chang-Whan Cho
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1011-1344)
- Published
- 2004
- Language
- EN
- Field
- Medicine (Physical Sciences)