About this Medicine article

Spectroscopic properties and photodynamic effects of new lipophilic porphyrin derivatives: Efficacy, localisation and cell death pathways by Gabriela Kramer-Marek; Carlos Serpa; Agnieszka Szurko; Maria Widel; Aleksander Sochanik; Miroslaw Snietura; Piotr Kus; Rui M.D. Nunes; Luis G. Arnaut; Alicja Ratuszna is a Medicine article available to read on EtoBox.

Photodynamic therapy (PDT) and photodynamic diagnostics (PDD) of cancer are based on the use of non-toxic dyes (photosensitisers) in combination with harmless visible light. This paper reports physicochemical properties, cell uptake, localisation as well as photodynamic efficiency of two novel lipophilic porphyrin derivatives, suitable for use as PDT sensitisers. Both compounds are characterised by high quantum yield of singlet oxygen generation which was measured by time-resolved phosphorescence. Photodynamic in vitro studies were conducted on three cancer cell lines. Results of cell survival tests showed negligible dark cytotoxicity but high phototoxicity. The results also indicate that cell death is dependent on energy dose and time following light exposure. Using confocal laser scanning microscopy both compounds were found to localise in the cytoplasm around the nucleus of the tumour cells. The mode of cell death was evaluated based on the morphological changes after differential staining. In summary, good photostability, high quantum yield of singlet oxygen and biological effectiveness indicate that the examined lipophilic porphyrin derivatives offer quite interesting prospect

Author

Gabriela Kramer-Marek; Carlos Serpa; Agnieszka Szurko; Maria Widel; Aleksander Sochanik; Miroslaw Snietura; Piotr Kus; Rui M.D. Nunes; Luis G. Arnaut; Alicja Ratuszna

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1011-1344)

Published

2006

Language

EN

Field

Medicine (Physical Sciences)