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Spontaneous production of a C‐type rna virus in rat tissue culture lines by Dr. V. Klement; M. O. Nicolson; W. Nelson-Rees; R. V. Gilden; S. Oroszlan; R. W. Ronoey; M. B. Gardner is a Medicine article available to read on EtoBox.

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## Abstract Recently we demonstrated the induction of a C‐type RNA virus (XCIV) in tissue culture line XC derived from Wistar random‐bred rats. In this article two other rat C‐type virus isolates (NRKAV and RPLAV) are described. They were associated with, and isolated from, NRK and RPL lines originating in Osborne‐Mendel and Lewis rat strains, respectively. The main biological characteristics shared by the three viruses were buoyant densities in sucrose gradients in the range of 1.14 to 1.15 g/ml, endogenous DNA polymerase activity, high molecular weight (60 to 70S) RNA, rat major speciesspecific (gs‐1) antigen and gs‐3 interspecies antigen. Two of the isolates (NRKAV and RPLAV) were able to rescue mouse sarcoma virus (MSV) genome from a rat MSV‐transformed non‐productive line. The NRK line was assayed for virus release at various in vitro passage levels. In lower passages (43–73) spontaneous virus release was absent or minimal but the virus could be induced by treatment of cells with 5‐bromodeoxyuridine. At higher passage levels the cells produced a large amount of virus spontaneously, but in these treatment with 5‐bromodeoxyuridine decreased virus production. The results of karyo

Who reads Spontaneous production of a C‐type rna virus in rat tissue culture lines?

Author

Dr. V. Klement; M. O. Nicolson; W. Nelson-Rees; R. V. Gilden; S. Oroszlan; R. W. Ronoey; M. B. Gardner

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1973

Language

EN

Field

Medicine (Health Sciences)