Can I read Opposite Effect of Oxidative Stress on Inducible Nitric Oxide Synthase and Haem Oxygenase-1 Expression in Intestinal Inflammation: Anti-inflammatory Effect of Carbon Monoxide on EtoBox?

Opposite Effect of Oxidative Stress on Inducible Nitric Oxide Synthase and Haem Oxygenase-1 Expression in Intestinal Inflammation: Anti-inflammatory Effect of Carbon Monoxide by Gerard Dijkstra; Hans Blokzijl; Lisette Bok; Manon Homan; Harry van Goor; Klaas Nico Faber; Peter LM Jansen; Han Moshage is a Medicine article available to read on EtoBox.

What is Opposite Effect of Oxidative Stress on Inducible Nitric Oxide Synthase and Haem Oxygenase-1 Expression in Intestinal Inflammation: Anti-inflammatory Effect of Carbon Monoxide about?

## Abstract Inducible nitric oxide synthase (iNOS) is expressed in intestinal epithelial cells (IEC) of patients with active inflammatory bowel disease (IBD) and in IEC of endotoxaemic rats. The induction of iNOS in IEC is an element of the NF‐κB‐mediated survival pathway. Haem oxygenase‐1 (HO‐1) is an AP‐1‐regulated gene that is induced by oxidative stress. The enzyme produces carbon monoxide (CO), which may attenuate the inflammatory response. The aim of this study was to investigate the regulation and interaction of iNOS and HO‐1 in response to inflammation and oxidative stress. Male Wistar rats were treated with the thiol‐modifying agent diethylmaleate (DEM) to induce oxidative stress and rendered endotoxaemic by LPS injection. Human colonic biopsies and the human colon carcinoma cell line DLD‐1 were treated with DEM and the lipid peroxidation end‐product 4‐hydroxynonenal to induce oxidative stress and exposed to cytokine mix (CM) to mimic inflammation. In some experiments, cells were incubated with 250–400 ppm CO prior to and during stimulation with CM. HO‐1 and iNOS expression was evaluated by RT‐PCR, western blotting, and immunohistology. NF‐κB activation was evaluated by EM

Who reads Opposite Effect of Oxidative Stress on Inducible Nitric Oxide Synthase and Haem Oxygenase-1 Expression in Intestinal Inflammation: Anti-inflammatory Effect of Carbon Monoxide?

Author

Gerard Dijkstra; Hans Blokzijl; Lisette Bok; Manon Homan; Harry van Goor; Klaas Nico Faber; Peter LM Jansen; Han Moshage

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

2004

Language

EN

Field

Medicine (Health Sciences)