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Cell Viability Assessment by Flow Cytometry Using Yeast as Cell Model by G. Mernier; N. Piacentini; R. Tornay; N. Buffi; P. Renaud is a Engineering article available to read on EtoBox.

What is Cell Viability Assessment by Flow Cytometry Using Yeast as Cell Model about?

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of a label-free, electrical device significantly decreases the system complexity and makes it more appropriate for use in point-of-care diagnostics. Living and dead yeast cells are separated by dielectrophoretic forces and counted using coulter counters. The combination of these two methods allows the determination of the percentage of living and dead cells for viability studies of cell samples. The device could further be used for sorting and counting of blood cells in applications such as diagnosis of insufficient cell concentrations, identification of cell deficiencies or bacterial contamination. The use of dielectrophoresis (DEP) as sorting principle allows to separate cells based on their dielectric properties in the place of size-based separation, enabling sorting of large panels of cells and separation of infected and non-infected cells of the same type.

Who reads Cell Viability Assessment by Flow Cytometry Using Yeast as Cell Model?

Author

G. Mernier; N. Piacentini; R. Tornay; N. Buffi; P. Renaud

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0925-4005)

Published

2011

Language

EN

Field

Engineering (Physical Sciences)