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This study compares three protein extraction protocols (TCA-acetone, phenol, and multi-detergent) for horse gram, a nutritionally important but under-studied legume. While all protocols yielded similar quantities of protein, the phenol method provided superior quality, as evidenced by better results in One-Dimensional and Two-Dimensional Electrophoresis. The developed protocol is applicable not only to horse gram but also to other leguminous and non-leguminous crops.

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madgamers1092

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EN