Can I read Sequence-based typing techniques for rhesus macaque MhcMamu-DQB1 allow the identification of more than 35 alleles on EtoBox?

Sequence-based typing techniques for rhesus macaque MhcMamu-DQB1 allow the identification of more than 35 alleles by N. Vigon; U. Sauermann is a Immunology and Microbiology article available to read on EtoBox.

What is Sequence-based typing techniques for rhesus macaque MhcMamu-DQB1 allow the identification of more than 35 alleles about?

**Abstract:** In the recent years, substantial progress has been made in the characterization of the rhesus macaque MHC region, which is more complex than in humans. To cope with the increasing knowledge, improved typing techniques for MHC genes are required. Including the DNA‐sequences in this report, 39 rhesus macaque (__Mamu__)__‐DQB1__ alleles, corresponding to 38 deduced protein sequences, are known. Here, we present a typing technique for __Mamu‐DQB1__ alleles and the DNA‐sequences of 9 novel __DQB1__ alleles. The technique consists of one or two rounds of screening followed by DNA‐sequence determination. The first round represents a low‐resolution screening sufficiently insensitive to identify novel alleles, which also allows sequencing of both alleles in most heterozygous individuals. The second round consists of a high‐resolution screening. This is only necessary for animals in which one PCR‐product was generated by the initial screening. The technique was validated by analyzing samples of more than 200 rhesus macaques of different origin, and DNA‐sequence determination of more than 230 PCR‐products.

Who reads Sequence-based typing techniques for rhesus macaque MhcMamu-DQB1 allow the identification of more than 35 alleles?

Author

N. Vigon; U. Sauermann

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0001-2815)

Published

2002

Language

EN

Field

Immunology and Microbiology (Life Sciences)