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Self-Incorporation of coenzymes by ribozymes by Ronald R. Breaker; Gerald F. Joyce is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Self-Incorporation of coenzymes by ribozymes about?

RNA molecules that are assembled from the four standard nucleotides contain a limited number of chemical functional groups, a characteristic that is generally thought to restrict the potential for catalysis by ribozymes. Although polypeptides carry a wider range of functional groups, many contemporary protein-based enzymes employ coenzymes to augment their capabilities. The coenzymes possess additional chemical moieties that can participate directly in catalysis and thereby enhance catalytic function. In this work, we demonstrate a mechanism by which ribozymes can supplement their limited repertoire of functional groups through RNAcatalyzed incorporation of various coenzymes and coenzyme analogues. The group I ribozyme of Tetrahymena thermophila normally mediates a phosphoester transfer reaction that results in the covalent attachment of guanosine to the ribozyme. Here, a shortened version of the ribozyme is shown to catalyze the self-incorporation of coenzymes and coenzyme analogues, such as NAD ÷ and dephosphorylated CoA-SH. Similar ribozyme activities may have played an important role in the "RNA world," when RNA enzymes are thought to have maintained a complex metabolism in the

Who reads Self-Incorporation of coenzymes by ribozymes?

Author

Ronald R. Breaker; Gerald F. Joyce

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0022-2844)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)