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Cloning and expression throughout mouse development of mfat1, a homologue of the Drosophila tumour suppressor gene fat by Barnaby Cox; Anna-Katerina Hadjantonakis; Jane E. Collins; Anthony I. Magee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Cloning and expression throughout mouse development of mfat1, a homologue of the Drosophila tumour suppressor gene fat about?

We present the entire sequence of the mouse Fat orthologue (mFat1), a protein of 4,588 amino acids with 34 cadherin repeats, 27 potential N-glycosylation sites, five EGF repeats and a laminin A G-motif in its extracellular domain. A single transmembrane region is followed by a cytoplasmic domain containing putative catenin-binding sequences. mFat1 shows high homology to human FAT and lesser homology to Drosophila Fat. The sequence of this giant cadherin suggests that it is unlikely to have a homophilic adhesive function, but may mediate heterophilic adhesion or play a signalling role. Expression analysis shows that the mfat1 gene is expressed early in pre-implantation mouse development, at the compact eight cell stage. Whole-mount and section in situ analyses show that transcripts are widely expressed throughout post-implantation development, most notably in the limb buds, branchial arches, forming somites, and in particular in the proliferating ventricular zones in the brain, being down-regulated as cells cease dividing. RT-PCR detects widespread expression in the adult suggesting a role in proliferation and differentiation of many tissues and cell types.

Who reads Cloning and expression throughout mouse development of mfat1, a homologue of the Drosophila tumour suppressor gene fat?

Author

Barnaby Cox; Anna-Katerina Hadjantonakis; Jane E. Collins; Anthony I. Magee

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)