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Contents page 1. General information S2 2. Experimental details and characterization data of new compounds S2 3. Materials and methods biology S14 4. Conjugation of trisaccharide 1 to CRM197 S14 5. Conjugation of trisaccharide 1 to HSA S15 6. Immunization of mice with the CRM197-1 glycoconjugate S16 7. ELISA S17 8. Immunofluorescence of the ultraviolet-inactivated H. pylori S17 9. Surface staining of H. pylori by flow cytometry S18 10. References S19 11. NMR Spectra of new compounds S20 S2 1. General Information. All reactions were performed with anhydrous solvents in oven-dried glassware with magnetic stirring under argon or nitrogen unless otherwise stated. The chemicals were used as supplied except where noted. Analytical thin layer chromatography (TLC) was conducted on precoated plates of silica gel (0.25-0.3 mm, Shanghai, China). The TLC plates were visualized by exposure to ultraviolet light or by staining with a sulfuric acid-ethanol solution. Silica gel column chromatography was performed on silica gel AR (100-200 mesh, Shanghai, China). Optical rotations (OR) were measured with a Rudolph Research Analytical Autopol I automatic polarimeter. NMR spectra were recorded with a

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American Chemical Society (ACS)