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This document summarizes RNA interference (RNAi) as a process by which double-stranded RNA inhibits gene expression. It discusses how RNAi works at a molecular level, including how long dsRNAs are processed by the enzyme Dicer into siRNAs that are incorporated into the RISC complex to target and cleave complementary mRNAs. It also describes how RNAi can be applied to mammalian cells using short siRNAs to avoid triggering an interferon response. Finally, it notes similarities and differences between RNAi and

Author

Liesbeth Allais

Language

EN