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Can I read High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor on EtoBox?
High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor by Ingo Knabben; Lars Regestein; Frank Marquering; Sven Steinbusch; Alvaro R. Lara; Jochen Büchs is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor about?
A common method to minimize overflow metabolism and to enable high cell-density is to operate microbial processes in fed-batch mode under carbon-limiting conditions. This requires sophisticated process control schemes with expensive hardware equipment and software and well-characterized processes parameters. To generate high-cell density, a more simplified strategy would be beneficial. Therefore, a genetically engineered Escherichia coli strain with a modified glucose uptake system was cultivated in batch mode. In the applied strain, the usual phosphotransferase system of a K12-derived strain was inactivated, while the galactose permease system was amplified. Upon cultivating this E. coli strain in pure minimal media, the acetate concentration did not exceed values of 0.35 g L -1 , even when the batch fermentation was started with a glucose concentration of 130 g L -1 . Finally, maximum biomass concentrations of 48 g L -1 dry cell weight and maximum space-time yields of 2.10 g L -1 h -1 were reached. To provide an unlimited growth under fully aerobic conditions (DOT > 30%) at comparatively low values for specific power input (3-4 kW m -3 ), a pressurized bioreactor was used. Conseq
Who reads High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ingo Knabben; Lars Regestein; Frank Marquering; Sven Steinbusch; Alvaro R. Lara; Jochen Büchs
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0168-1656)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)