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Overexpression of Endoplasmic Reticulum Omega-3 Fatty Acid Desaturase Gene Improves Chilling Tolerance in Tomato by Chao Yu; Hua-Sen Wang; Sha Yang; Xian-Feng Tang; Ming Duan; Qing-Wei Meng is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Overexpression of Endoplasmic Reticulum Omega-3 Fatty Acid Desaturase Gene Improves Chilling Tolerance in Tomato about?

An endoplasmic reticulum-localized tomato omega-3 fatty acid desaturase gene (LeFAD3) was isolated and characterized with regard to its sequence, response to various temperatures and function in transgenic tomato plants. Northern blot analysis showed that LeFAD3 was expressed in all organs tested and was markedly abundant in roots. Meanwhile, the expression of LeFAD3 was induced by chilling stress (4 degrees C), but inhibited by high temperature (40 degrees C). The transgenic plants were obtained under the control of the cauliflower mosaic virus 35S promoter (35S-CaMV). Northern and western blot analyses confirmed that sense LeFAD3 was transferred into tomato genome and overexpressed. Level of linolenic acids (18:3) increased and correspondingly level of linoleic acid (18:2) decreased in leaves and roots. After chilling stress, the fresh weight of the aerial parts of transgenic plants was higher than that of the wild type (WT) plants, and the membrane system ultrastructure of chloroplast in leaf cell and all the subcellular organelles in root tips of transgenic plants kept more intact than those of WT. Relative electric conductivity increased less in transgenic plants than that in

Who reads Overexpression of Endoplasmic Reticulum Omega-3 Fatty Acid Desaturase Gene Improves Chilling Tolerance in Tomato?

Author

Chao Yu; Hua-Sen Wang; Sha Yang; Xian-Feng Tang; Ming Duan; Qing-Wei Meng

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0981-9428)

Published

2009

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)