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What is Resuspension of Bacterial Pellets in DNA Miniprep about?
Students will isolate plasmid DNA from E. coli cultures using a miniprep kit. They will then digest the plasmids with restriction enzymes and run them on a gel to check for correctly sized inserts. Finally, they will determine DNA concentrations and send samples for sequencing to verify the construct sequences.
- Author
- sunny993
- Language
- EN