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Development of a Fibrin Composite-coated Poly(ε-caprolactone) Scaffold for Potential Vascular Tissue Engineering Applications by Divya Pankajakshan; Lizymol P Philipose; Minshiya Palakkal; Kalliyana Krishnan; Lissy K. Krishnan is a scholarly article available to read on EtoBox.

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## Abstract Poor cell adhesion, cytotoxicity of degradation products and lack of biological signals for cell growth, survival, and tissue generation are the limitations in the use of a biodegradable polymer scaffold for vascular tissue engineering. We have fabricated a hybrid scaffold by integrating physicochemical characteristics of poly(ε‐caprolactone) (PCL) and biomimetic property of a composite of fibrin, fibronectin, gelatin, growth factors, and proteoglycans to improve EC growth on the scaffold. Solvent cast porous films of poly(ε‐caprolactone) was prepared using PEG as a porogen. Porosity varied between 5 and 200 μm, and FTIR spectroscopy confirmed structural aspects of PCL. Films kept in PBS for 60 days showed tensile strength and elongation matching native blood vessel. Slow degradation of the scaffold was demonstrated by gravimetric analysis and molecular weight determination. Human umbilical vein endothelial cell (HUVEC) adhesion and proliferation on bare films were minimal. FTIR spectroscopy and environmental scanning electron microscopy (ESEM) of PCL‐fibrin hybrid scaffold confirmed the presence of fibrin composite on PCL film. HUVEC was subsequently cultured on hybrid

Author

Divya Pankajakshan; Lizymol P Philipose; Minshiya Palakkal; Kalliyana Krishnan; Lissy K. Krishnan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1552-4973)

Published

2008

Language

EN