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A Simplified Polyacrylamide Gel Electrophoresis Apparatus for Simultaneous Application of Multiple Buffer Systems or Detergent Combinations by Andrew C. Newby; Gary Matthews; Andreas Chrambach is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A Simplified Polyacrylamide Gel Electrophoresis Apparatus for Simultaneous Application of Multiple Buffer Systems or Detergent Combinations about?

A previous design of an apparatus for the simultaneous fractionation by polyacrylamide gel electrophoresis in 10 different buffer systems (1) was replaced by a greatly simplified new design, employing small, cylindrical buffer partitions within the lower buffer reservoir and/or upper buffer reservoir of a conventional, temperature-regulated polyacrylamide gel electrophoresis apparatus for cylindrical gels. The apparatus was tested in application to the problem of simultaneous polyacrylamide gel electrophoresis in different buffer systems with the purpose of optimizing the operative pH for a particular fractionation problem. It was also applied to fractionations in a single buffer system to which various combinations of ionic and nonionic detergents were admixed.

Who reads A Simplified Polyacrylamide Gel Electrophoresis Apparatus for Simultaneous Application of Multiple Buffer Systems or Detergent Combinations?

Author

Andrew C. Newby; Gary Matthews; Andreas Chrambach

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1978

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)