Opening book details…
Can I read Macrophage-targeting oligopeptides from Mortierella alpina † on EtoBox?
Macrophage-targeting oligopeptides from Mortierella alpina † by Jacob M. Wurlitzer; Aleksa Stanišić; Sebastian Ziethe; Paul M. Jordan; Kerstin Günther; Oliver Werz; Hajo Kries; Markus Gressler is a Chemistry article available to read on EtoBox.
What is Macrophage-targeting oligopeptides from Mortierella alpina † about?
The realm of natural products of early diverging fungi such as __Mortierella__ species is largely unexplored. Herein, the nonribosomal peptide synthetase (NRPS) MalA catalysing the biosynthesis of the surface-active biosurfactants, malpinins, has been identified and biochemically characterised. The investigation of the substrate specificity of respective adenylation (A) domains indicated a substrate-tolerant enzyme with an unusual, inactive C-terminal NRPS module. Specificity-based precursor-directed biosynthesis yielded 20 new congeners produced by a single enzyme. Moreover, MalA incorporates artificial, click-functionalised amino acids which allowed postbiosynthetic coupling to a fluorophore. The fluorescent malpinin conjugate penetrates mammalian cell membranes __via__ an phagocytosis-mediated mechanism, suggesting __Mortierella__ oligopeptides as carrier peptides for directed cell targeting. The current study demonstrates substrate-specificity testing as a powerful tool to identify flexible NRPS modules and highlights basal fungi as reservoir for chemically tractable compounds in pharmaceutical applications.
Who reads Macrophage-targeting oligopeptides from Mortierella alpina †?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Jacob M. Wurlitzer; Aleksa Stanišić; Sebastian Ziethe; Paul M. Jordan; Kerstin Günther; Oliver Werz; Hajo Kries; Markus Gressler
- Published
- 2022
- Language
- EN
- Field
- Chemistry (Physical Sciences)