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Expression of the Sodium Iodide Symporter in Human Kidney by Christine Spitzweg; Charyl M. Dutton; Maria R. Castro; Elizabeth R. Bergert; John R. Goellner; Armin E. Heufelder; John C. Morris is a Medicine article available to read on EtoBox.

What is Expression of the Sodium Iodide Symporter in Human Kidney about?

## Background: The human sodium iodide symporter (hnis) is a transmembrane protein that mediates the active transport of iodide in the thyroid gland. following cloning of nis, nis expression has been detected in a broad range of nonthyroidal tissues, suggesting that iodide transport in these tissues is conferred by the expression of functional nis protein. ## Methods: The aim of this study was to examine functional hnis expression in kidney by reverse transcription-polymerase chain reaction (rt-pcr), ribonuclease protection assay (rpa), immunohistochemistry, and western blot analysis accompanied by iodide accumulation studies in kidney cells. ## Results: Using a pair of full-length hnis-specific oligonucleotide primers, rt-pcr followed by southern hybridization revealed hnis mrna expression in normal human kidney tissue. the pcr products were subjected to automated sequencing and revealed full identity with the published human thyroid-derived nis cdna sequence. furthermore, positive protected bands indicating the presence of hnis mrna were apparent in rpa gel lanes corresponding to human kidney cells as well as chinese hamster ovary (cho) cells stably transfected with hnis cdna and

Who reads Expression of the Sodium Iodide Symporter in Human Kidney?

Author

Christine Spitzweg; Charyl M. Dutton; Maria R. Castro; Elizabeth R. Bergert; John R. Goellner; Armin E. Heufelder; John C. Morris

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; ScienceOpen (ISSN 0085-2538)

Published

2001

Language

EN

Field

Medicine (Health Sciences)