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Can I read Cotranscriptional R-loop formation by Mfd involves topological partitioning of DNA on EtoBox?
Cotranscriptional R-loop formation by Mfd involves topological partitioning of DNA by James R. Portman; Gwendolyn M. Brouwer; Jack Bollins; Nigel J. Savery; Terence R. Strick is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Cotranscriptional R-loop formation by Mfd involves topological partitioning of DNA about?
## Significance R-loop structures pose a threat to genomic integrity because their formation can lead to mutagenesis. Mutagenesis drives antibiotic resistance in bacteria and chemotherapy resistance in human cancers; therefore, it is important to understand how R-loops form. Using a combination of in vitro single-molecule experimentation and in vivo mutagenesis assays, we have shown how Mfd, a bacterial protein known for its role in DNA repair, can interact with RNA polymerase to form R-loops. This interaction generates a topological domain in DNA that is highly prone to R-loop formation. The observed mechanism relies on properties of Mfd that are shared by many other proteins, hinting that this is a potentially universal model for R-loop formation.
Who reads Cotranscriptional R-loop formation by Mfd involves topological partitioning of DNA?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- James R. Portman; Gwendolyn M. Brouwer; Jack Bollins; Nigel J. Savery; Terence R. Strick
- Publisher
- National Academy of Sciences
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)