About this Agricultural and Biological Sciences article

Novel Stripe Rust Effector Boosts the Transcription of a Host Susceptibility Factor Through Affecting Histone Modification to Promote Infection in Wheat by Wanlu Duan; Zhenkai Hao; Huihui Pang; Yuxi Peng; Yiwen Xu; Yanfei Zhang; Ying Zhang; Zhensheng Kang; Jing Zhao is a Agricultural and Biological Sciences article available to read on EtoBox.

## Summary Regulation of host gene expression to promote disease is a common strategy for plant pathogens. However, it remains unclear whether or not fungal pathogens manipulate host gene expression directly through secreted effectors with transcriptional activity. Here, we identified a fungal effector PstGTA1 from __Puccinia striiformis__ f. sp. __tritici__ (__Pst__), which has partial homology to the subunit of global transcriptional activator SNF2 from oyster. The transcriptional activating activity of PstGTA1 was validated in yeast, and the potential role of PstGTA1 in pathogenicity was assessed using gene silenced and overexpression transgenic wheat plants. Candidate targets regulated by PstGTA1 were screened by transcriptomic analysis, and the specific promoter region binding to PstGTA1 was further determined. PstGTA1 can be delivered to the wheat cell nucleus and contributes to the full virulence of __Pst__ by targeting the promoter of __TaSIG__, a gene negatively regulating wheat immunity, and possibly activates its transcription by affecting the histone H3K4 acetylation level. Our study provides the first direct evidence for a fungal effector with transactivation activity

Author

Wanlu Duan; Zhenkai Hao; Huihui Pang; Yuxi Peng; Yiwen Xu; Yanfei Zhang; Ying Zhang; Zhensheng Kang; Jing Zhao

Publisher

Wiley

Published

2023

Field

Agricultural and Biological Sciences (Life Sciences)