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Comparison of various atmospheric conditions for isolation and subcultivation of Mycoplasma hyorhinis from cell cultures by Anna A. Polak-Vogelzang; H. H. de Haan; J. Borst is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The efficiency of aerobic incubation was compared with incubation under various oxygen and carbon dioxide conditions for the isolation and subcultivation of three strains of Mycoplasma hyorhinis from VERO-cell cultures and subcultivation of three laboratory strains. Under anaerobic conditions with a low oxidation-reduction potential (at or below -115 mV) as obtained in jars, with catalysts, containing mixtures of 5%-10% CO2 in H2, very poor or no growth of any of the six M. hyorhinis strains was observed. When traces of oxygen were present (that is, under conditions with higher oxidation-reduction potentials, e.g. when omitting the catalyst in the above gas mixtures or in 5% CO2 + 95% N2) isolation from cell cultures was successful in most tests, but subcultivation of these primary isolates was seldom possible under these semi-anaerobic conditions. However, in most cases these primary isolates could be subcultivated aerobically, although aerobic conditions were unsatisfactory for isolation in about half of the experiments. Isolation of M. hyorhinis was optimal in 5% O2 + 95% N2, under which condition the isolates could also always be subcultivated. Isolation failed occasionally whe

Who reads Comparison of various atmospheric conditions for isolation and subcultivation of Mycoplasma hyorhinis from cell cultures?

Author

Anna A. Polak-Vogelzang; H. H. de Haan; J. Borst

Publisher

Springer Netherlands; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0003-6072)

Published

1983

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)