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Can I read Effect of organic solvents on cell-bound penicillin V acylase activity of Erwinia aroideae (DSMZ 30186): A permeabilization effect on EtoBox?
Effect of organic solvents on cell-bound penicillin V acylase activity of Erwinia aroideae (DSMZ 30186): A permeabilization effect by Atul Kumar; Archana Pundle is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Effect of organic solvents on cell-bound penicillin V acylase activity of Erwinia aroideae (DSMZ 30186): A permeabilization effect about?
Erwinia aroideae (DSMZ 30186) is a potential microbial culture to produce intracellular penicillin V acylase (PVA). The whole cell PVA activity was improved by permeabilization with various organic solvents. The cell-bound PVA activity showed an eightfold increase upon treatment with chloroform (5 L/mg dry biomass ) for 10 min and diethyl ether (10 L/mg dry biomass ) for 45 min. Hexane, toluene, ethyl acetate and dichloromethane enhanced the enzyme activity up to two-, six-, four-and two-fold, respectively; whereas, PVA activity declined drastically on permeabilization with acetone, pyridine and alcohols. The physicochemical properties of the organic solvents used for permeabilization were correlated with the change in activity. It was found that solvents with high hydrophobicity (log P > 0.68) and lower dielectric constant (<9) were relatively effective in increasing PVA activity. These results allow systematic selection of suitable solvent for best performance.
Who reads Effect of organic solvents on cell-bound penicillin V acylase activity of Erwinia aroideae (DSMZ 30186): A permeabilization effect?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Atul Kumar; Archana Pundle
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1381-1177)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)