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Measurement of aqueous glucose in a model anterior chamber using Raman spectroscopy by James L. Lambert; John Michael Morookian; Shannon J. Sirk; Mark S. Borchert is a Chemistry article available to read on EtoBox.

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## Abstract Many laboratories have been developing techniques that might be applied toward a non‐invasive device that could optically measure aqueous humor glucose in the eye as a surrogate for blood glucose. We have configured a confocal Raman microscope that is capable of acquiring the Raman spectra of the aqueous humor __in vivo__. However, because of the potential for optical toxicity, a major design consideration is evaluating the tradeoff between the energy of exposure and measurement accuracy. Toward this end, we have developed a physical model of the anterior chamber in the eye for studying the feasibility of using a confocal Raman microscope for determining the concentration of glucose and other metabolites. The Raman spectrum of aqueous humor closely resembles the spectrum of its four primary Raman scatterers, (glucose, urea, lactate and ascorbate) mixed in normal saline. Aqueous mixtures of these analytes were placed under a contact lens resting on a quartz plate to form a physical model of the anterior chamber filled with aqueous humor. Calibration standards were prepared by non‐collinearly varying the measured concentrations of the analytes over a range of 0–13 times t

Who reads Measurement of aqueous glucose in a model anterior chamber using Raman spectroscopy?

Author

James L. Lambert; John Michael Morookian; Shannon J. Sirk; Mark S. Borchert

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0377-0486)

Published

2002

Language

EN

Field

Chemistry (Physical Sciences)