Can I read Colon carcinoma glycoproteins carrying α 2,6-linked sialic acid reactive withSambucus Nigra agglutinin are not constitutively expressed in normal human colon mucosa and are distinct from sialyl-tn antigen on EtoBox?

Colon carcinoma glycoproteins carrying α 2,6-linked sialic acid reactive withSambucus Nigra agglutinin are not constitutively expressed in normal human colon mucosa and are distinct from sialyl-tn antigen by Toshihiko Murayama; Christian Zuber; Walter K.F. Seelentag; Wei-Ping Li; Wolfgang Kemmner; Philipp U. Heitz; Jürgen Roth is a Medicine article available to read on EtoBox.

What is Colon carcinoma glycoproteins carrying α 2,6-linked sialic acid reactive withSambucus Nigra agglutinin are not constitutively expressed in normal human colon mucosa and are distinct from sialyl-tn antigen about?

In human colon carcinoma, increased amounts of sialic acids have been found and correlated with tumor progression. Further, the degree of O-acetylation of sialic acid residues in normal mucosa is higher than in colon carcinoma. Thus, tumor-associated sialylated antigens may be constitutively expressed in O-acetylated form in normal mucosa unreactive with the respective monoclonal antibodies. We have earlier demonstrated a colon carcinoma-associated expression of a 2,6-linked sialic acid residues with the Sambucus nigra agglutinin (SNA). We report now that de-acetylation of normal and transitional colonic mucosa, in contrast to sialyl-Tn antigen, does not result in SNA binding. Further, the a 2,6-linked sialic acid recognized by SNA is distinct from that of sialyl-Tn antigen. This is confirmed by Northern blotting detecting transcripts for a 2,6 sialyltransferase of N-glycoproteins and measurement of activity for this sialyltransferase. Blot analysis by SNA of colon carcinoma cells revealed few reactive glycoproteins. Quantitative differences in lectin labeling and sialyltransferase activity were found in HCT116 colon carcinoma cell sub-lines. Our data suggest that SNA binding in hu

Who reads Colon carcinoma glycoproteins carrying α 2,6-linked sialic acid reactive withSambucus Nigra agglutinin are not constitutively expressed in normal human colon mucosa and are distinct from sialyl-tn antigen?

Author

Toshihiko Murayama; Christian Zuber; Walter K.F. Seelentag; Wei-Ping Li; Wolfgang Kemmner; Philipp U. Heitz; Jürgen Roth

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1997

Language

EN

Field

Medicine (Health Sciences)