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Analysis of the Contribution of Sedimentation to Bacterial Mass Transport in a Parallel Plate Flow Chamber by Jiuyi Li; Henk J. Busscher; Henny C. van der Mei; Willem Norde; Bastiaan P. Krom; Jelmer Sjollema is a Materials Science article available to read on EtoBox.

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Using a new phase-contrast microscopy-based method of analysis, sedimentation has recently been demonstrated to be the major mass transport mechanism of bacteria towards substratum surfaces in a parallel plate flow chamber (J. Li, H.J. Busscher, W. Norde, J. Sjollema, Colloid Surf. B. 84 (2011) 76). Here we describe a novel method for enumerating adhesion of fluorescent bacteria in a parallel plate flow chamber that allows direct imaging of the bacterial distribution along the length of the flow chamber, as caused by sedimentation. Imaging of fluorescence was done using macroscopic bio-optical imaging of the entire flow chamber, including top and bottom plates as well as of the flowing suspension in between. An algorithm is forwarded that allows to separate the fluorescence arising from the suspension and bottom plate and at the same time determines the single cell fluorescence from which the bacterial distribution over the entire bottom plate can be visualized. Enumeration of the numbers of bacteria adhering to the center of the glass bottom plate for a fluorescent Staphylococcus aureus strain was found to coincide with enumerations using phase-contrast microscopy. Moreover, due t

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Author

Jiuyi Li; Henk J. Busscher; Henny C. van der Mei; Willem Norde; Bastiaan P. Krom; Jelmer Sjollema

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0927-7765)

Published

2011

Language

EN

Field

Materials Science (Physical Sciences)