Can I read Tyrosine Phosphorylations Specific to Mitosis in Human and Hamster Cells on EtoBox?

Tyrosine Phosphorylations Specific to Mitosis in Human and Hamster Cells by Robert Schlegel; M. Olivia Harris; Glenn S. Belinsky is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Tyrosine Phosphorylations Specific to Mitosis in Human and Hamster Cells about?

Changes in protein tyrosine phosphorylation are known to be important for regulating cell cycle progression. With the aim of identifying new proteins involved in the regulation of mitosis, we used an antibody against phosphotyrosine to analyze proteins from synchronized human and hamster cells. At least seven proteins were found that displayed mitosis-specific tyrosine phosphorylation in HeLa cells (~~165,205,240, 250, 270, 290, and -400) and one such protein in hamster BHK cells (ppl55). In synchronized HeLa and BHK cells, all proteins except HeLa pp165, pp205, and pp250 were readily detectable only in mitosis. Tyrosine phosphorylation of pp165, pp205, and pp250 was apparent during arrest in S phase, suggesting that cell cycle perturbations can affect the phosphorylation state of some of these proteins. In a related finding in BHK cells, ppl55 underwent tyrosine phosphorylation when cells were forced into premature mitosis by caffeine treatment. Only one protein (pp135 in HeLa cells) was found to be dephosphorylated on tyrosine during mitosis. The above findings may prove helpful for isolating new cell cycle proteins that are important for both the normal regulation of mitosis and

Who reads Tyrosine Phosphorylations Specific to Mitosis in Human and Hamster Cells?

Author

Robert Schlegel; M. Olivia Harris; Glenn S. Belinsky

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)