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Characterizing Silicone Oil-Induced Protein Aggregation with Stimulated Raman Scattering Imaging by Brian Wong; Xi Zhao; Yongchao Su; Hanlin Ouyang; Timothy Rhodes; Wei Xu; Hanmi Xi; Dan Fu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Characterizing Silicone Oil-Induced Protein Aggregation with Stimulated Raman Scattering Imaging about?
Particles in biopharmaceutical products present high risks due to their detrimental impacts on product quality and safety. Identification and quantification of particles in drug products are important to understand particle formation mechanisms, which can help develop control strategies for particle formation during the formulation development and manufacturing process. However, existing analytical techniques such as microflow imaging and light obscuration measurement lack the sensitivity and resolution to detect particles with sizes smaller than 2 μm. More importantly, these techniques are not able to provide chemical information to determine particle composition. In this work, we overcome these challenges by applying the stimulated Raman scattering (SRS) microscopy technique to monitor the C-H Raman stretching modes of the proteinaceous particles and silicone oil droplets formed in the prefilled syringe barrel. By comparing the relative signal intensity and spectral features of each component, most particles can be classified as protein-silicone oil aggregates. We further show that morphological features are poor indicators of particle composition. Our method has the capability t
Who reads Characterizing Silicone Oil-Induced Protein Aggregation with Stimulated Raman Scattering Imaging?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Brian Wong; Xi Zhao; Yongchao Su; Hanlin Ouyang; Timothy Rhodes; Wei Xu; Hanmi Xi; Dan Fu
- Publisher
- American Chemical Society (ACS)
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)