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Can I read Transcriptome characterization and polymorphism detection between subspecies of big sagebrush ( Artemisia tridentata ) on EtoBox?
Transcriptome characterization and polymorphism detection between subspecies of big sagebrush ( Artemisia tridentata ) by Prabin Bajgain; Bryce A Richardson; Jared C Price; Richard C Cronn; Joshua A Udall is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Transcriptome characterization and polymorphism detection between subspecies of big sagebrush ( Artemisia tridentata ) about?
## Background Big sagebrush (__Artemisia tridentata__) is one of the most widely distributed and ecologically important shrub species in western North America. This species serves as a critical habitat and food resource for many animals and invertebrates. Habitat loss due to a combination of disturbances followed by establishment of invasive plant species is a serious threat to big sagebrush ecosystem sustainability. Lack of genomic data has limited our understanding of the evolutionary history and ecological adaptation in this species. Here, we report on the sequencing of expressed sequence tags (ESTs) and detection of single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) markers in subspecies of big sagebrush. ## Results cDNA of __A. tridentata__ sspp. __tridentata__ and __vaseyana__ were normalized and sequenced using the 454 GS FLX Titanium pyrosequencing technology. Assembly of the reads resulted in 20,357 contig consensus sequences in ssp. __tridentata__ and 20,250 contigs in ssp. __vaseyana__. A BLASTx search against the non-redundant (NR) protein database using 29,541 consensus sequences obtained from a combined assembly resulted in 21,436 sequences with sig
Who reads Transcriptome characterization and polymorphism detection between subspecies of big sagebrush ( Artemisia tridentata )?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Prabin Bajgain; Bryce A Richardson; Jared C Price; Richard C Cronn; Joshua A Udall
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2000-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2164)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)