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Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells by Zhenyu Chen; Huaijin Liu; Jingjing Zhu; Xing Duan; Han Wang; Xiangchen Li; Xiaolong Zhou; Ayong Zhao; Songbai Yang is a Medicine article available to read on EtoBox.

What is Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells about?

## Background Promyelocytic leukemia protein (PML) is a primary component of PML nuclear bodies (PML-NBs). PML and PML-NBs play critical roles in processes like the cell cycle, DNA damage repair, apoptosis, and the antiviral immune response. Previously, we identified five porcine PML alternative splicing variants and observed an increase in the expression of these PML isoforms following Japanese encephalitis virus (JEV) infection. In this study, we examined the functional roles of these PML isoforms in JEV infection. ## Methods PML isoforms were either knocked down or overexpressed in PK15 cells, after which they were infected with JEV. Subsequently, we analyzed the gene expression of PML isoforms, JEV, and the interferon (IFN)-β signaling pathway using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot. Viral titers were determined through 50% tissue culture infectious dose (TCID~50~) assays. ## Results Our results demonstrated that the knockdown of endogenous PML promoted JEV replication, while the overexpression of PML isoforms 1, 3, 4, and 5 (PML1, PML3, PML4, and PML5) inhibited JEV replication. Further investigation revealed that PML1, PML

Who reads Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells?

Author

Zhenyu Chen; Huaijin Liu; Jingjing Zhu; Xing Duan; Han Wang; Xiangchen Li; Xiaolong Zhou; Ayong Zhao; Songbai Yang

Publisher

Springer Science and Business Media LLC

Published

2023

Field

Medicine (Health Sciences)