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Fibrinogen Induces Chemotactic Activity in Endothelial Cells by F. H. Seeger; E. Blessing; L. Gu; R. Bornhold; S. Denger; J. Kreuzer is a Medicine article available to read on EtoBox.

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## ABSTRACT Chemotaxis of blood monocytes into the vessel wall together with the change of the relative content of the extracellular matrix (ECM) proteins at sites of predilection is an early cellular marker of atherogenesis. To examine the influence of ECM proteins on secretion of chemoattractants by endothelial cells (EC), porcine EC were seeded on gelatin (G), fibronectin (Fn) and fibrinogen (Fg). After 24 h cells seeded on G and Fn showed the histiotypic ‘cobblestone’‐morphology whereas cells seeded on Fg did not. Chemotactic activity for monocytes in supernatants from cells seeded on Fg was more than two‐fold higher compared with G and was independent of soluble Fn or Fg in the supernatant. Quantification of monocyte chemoattracting protein‐1, PDGF‐AB and IL‐8 in EC supernatants showed that Fg led to a significant increase in secretion of all three proteins compared with cells cultured on G. Preincubation of porcine EC with the tripeptide arginine–glycine–aspartic acid, as inhibitor of binding of Fg to integrin receptors, but not with the control tripeptide arginine–glycine–glutamic acid showed a decrease in chemotactic activity for cells cultured on Fg but not on Fn or G. Inh

Who reads Fibrinogen Induces Chemotactic Activity in Endothelial Cells?

Author

F. H. Seeger; E. Blessing; L. Gu; R. Bornhold; S. Denger; J. Kreuzer

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0001-6772)

Published

2002

Language

EN

Field

Medicine (Life Sciences)