About this document
Enhanced Bio-Isoprene Production in E. coli by d.ubi is a document available to read on EtoBox.
This study demonstrates the production of isoprene by engineered Escherichia coli using the exogenous mevalonate pathway, achieving a maximum yield of 1832 mg/L through codon optimization and gene deletions to reduce byproducts. The research highlights the effectiveness of combining the isoprene synthase gene from Populus trichocarpa with a high-copy plasmid and strong promoter to enhance production. The findings suggest a promising biological alternative to petroleum-based isoprene, addressing sustainabili
- Author
- d.ubi
- Language
- EN