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Molecular characteristics, polymorphism and expression analysis of mhc II in yellow catfish( pelteobagrus fulvidraco )responding to Flavobacterium columnare infection by Bingchao Wang,Fangzheng Zhu,Zechao Shi,Zhenyu Huang,Ruhan Sun,Qingchao Wang,Gang Ouyang,Wei Ji is a nonfiction available to read on EtoBox.

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Fish and Shellfish Immunology, 125 (2022) 90-100. doi:10.1016/j.fsi.2022.04.036 Molecular characteristics, polymorphism and expression analysis of mhc II in yellow catfish(pelteobagrus fulvidraco)respondi ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Fish collection, bacterial challenging and sampling 2 2.2 RNA extraction and cDNA preparation 2 2.3 Molecular cloning of mhc IIA and mhc IIB genes 2 2.4 Sequence analysis 3 2.5 Quantitative real-time PCR and expression analysis 3 2.6 Histology and light microscopy study 3 2.7 Statistics 3 3 Results 4 3.1 Characterization of mhc IIA and mhc IIB genes 4 3.2 Molecular polymorphism of mhc IIA and mhc IIB genes 5 3.3 Phylogenetic analysis of Mhc IIA and Mhc IIB genes 6 3.4 Tissue distribution of mhc IIA and mhc IIB genes 6 3.5 The expression of mhc IIA and mhc IIB during early embryonic developmental stages 6 3.6 Morphological changes of gills in yellow catfish after challenged with F. columnare 6 3.7 Expression of mhc IIA and mhc IIB genes in response to F. columnare challenge 6 4 Discussion 7 CRediT authorship contribution statement 10 Acknowledgments 10 References 10 Yellow catfish (Pelteobagrus fulvidraco),mhc II,Polymorphism,Gen

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Author
Bingchao Wang,Fangzheng Zhu,Zechao Shi,Zhenyu Huang,Ruhan Sun,Qingchao Wang,Gang Ouyang,Wei Ji
Publisher
Elsevier Ltd
Language
EN
Category
nonfiction

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