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Quantitative Profiling of Epoxyeicosatrienoic, Hydroxyeicosatetraenoic, and Dihydroxyeicosatetraenoic Acids in Human Intrauterine Tissues Using Liquid Chromatography/electrospray Ionization Tandem Mass Spectrometry by Ji-Hong Zhang; Timothy Pearson; Balwir Matharoo-Ball; Catharine A. Ortori; Averil Y. Warren; Raheela Khan; David A. Barrett is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A reversed-phase liquid chromatography negative ion electrospray tandem mass spectrometry (LC/ESI-MS/MS) method was developed and validated to quantify a range of physiologically relevant eicosanoids, including 5, 8,11,and 14,and 5,8,and 12, in human intrauterine tissues. A solid-phase extraction method was employed to extract the eicosanoids, and gradient LC separation was performed on a Kromasil C 18 column. Mass spectrometric detection was performed by multiple reaction monitoring over a 31-min run time. The calibration curves were linear over the range of 4-400 pmol/g tissue, and the intra-and interday precision and accuracy were within a coefficient of variation of 2.0 to 27.4% and 4.6 to 17.9%, respectively. The lower limit of quantitation was 1.0 pmol/g tissue. The method was applied successfully to the characterization and quantitation of eicosanoids in the different compartments of human intrauterine tissues. Our results demonstrate significantly greater amounts of HETEs than of either the EETs or DiHETEs (P < 0.001), irrespective of tissue type. Specifically, the metabolite 12-HETE was significantly more abundant (P < 0.001) than all other HETEs. Of the EET metabolites, 5

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Author

Ji-Hong Zhang; Timothy Pearson; Balwir Matharoo-Ball; Catharine A. Ortori; Averil Y. Warren; Raheela Khan; David A. Barrett

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)