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Can I read Structure–function Relationships in Spruce Budworm Antifreeze Protein Revealed by Isoform Diversity on EtoBox?
Structure–function Relationships in Spruce Budworm Antifreeze Protein Revealed by Isoform Diversity by Daniel Doucet; Michael G. Tyshenko; Michael J. Kuiper; Steffen P. Graether; Brian D. Sykes; Andrew J. Daugulis; Peter L. Davies; Virginia K. Walker is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Structure–function Relationships in Spruce Budworm Antifreeze Protein Revealed by Isoform Diversity about?
The spruce budworm, __Choristoneura fumiferana__, produces antifreeze protein (AFP) to assist in the protection of the overwintering larval stage. AFPs are thought to lower the freezing point of the hemolymph, noncolligatively, by interaction with the surface of ice crystals. Previously, we had identified a cDNA encoding a 9‐kDa AFP with 10–30 times the thermal hysteresis activity, on a molar basis, than that shown by fish AFPs. To identify important residues for ice interaction and to investigate the basis for the hyperactivity of the insect AFPs, six new spruce budworm AFP cDNA isoforms were isolated and sequenced. They differ in amino‐acid identity as much as 36% from the originally characterized AFP and can be divided into three classes according to the length of their 3′ untranslated regions (UTRs). The new isoforms have at least five putative ‘Thr‐X‐Thr’ ice‐binding motifs and three of the new isoforms encode larger, 12‐kDa proteins. These appear to be a result of a 30 amino‐acid insertion bearing two additional ice‐binding motifs spaced 15 residues apart. Molecular modeling, based on the NMR structure of a short isoform, suggests that the insertion folds into two additional
Who reads Structure–function Relationships in Spruce Budworm Antifreeze Protein Revealed by Isoform Diversity?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Daniel Doucet; Michael G. Tyshenko; Michael J. Kuiper; Steffen P. Graether; Brian D. Sykes; Andrew J. Daugulis; Peter L. Davies; Virginia K. Walker
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)