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Platelet and Monoclonal Antibody Binding to Fibrinogen Adsorbed on Glow‐discharge‐deposited Polymers by David Kiaei; Allan S. Hoffman; Thomas A. Horbett; Ken R. Lew is a Medicine article available to read on EtoBox.

What is Platelet and Monoclonal Antibody Binding to Fibrinogen Adsorbed on Glow‐discharge‐deposited Polymers about?

The state of fibrinogen adsorbed on untreated and glowdischarge-treated surfaces was examined by measuring platelet adhesion, monoclonal antibody (mAb) binding, the amount of fibrinogen adsorbed, and the amount of adsorbed fibrinogen which could be eluted with sodium dodecyl sulfate (SDS). Tetrafluoroethylene (TFE) glowdischarge-treated polymers have a lower surface free energy (in air) and retain a larger fraction of adsorbed fibrinogen than untreated surfaces after SDS elution. Platelet adhesion was lowest on the TFE-treated surfaces which retain the highest amounts of fibrinogen after SDS elution. Fibrinogen may undergo unfolding or spreading on the TFE-treated surfaces to minimize interfacial free energy (in water) and maximize protein-surface interactions. When it is adsorbed on the TFE-treated surfaces, fibrinogen evidently assumes a state which somehow prevents its recognition and binding by platelet receptors. Monoclonal antibodies that bind to the three regions in fibrinogen thought to be involved in platelet adhesion were therefore used to detect changes in adsorbed fibrinogen. These regions and the antibodies which bind to them are: the COOH-terminal of the y-chain, mAb

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Author

David Kiaei; Allan S. Hoffman; Thomas A. Horbett; Ken R. Lew

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Heterocorporation; Wiley (ISSN 0021-9304)

Published

1995

Language

EN

Field

Medicine (Health Sciences)