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New conditions for matrix-assisted laser desorption/ionization mass spectrometry of native bacterial R-type lipopolysaccharides by Luisa Sturiale; Domenico Garozzo; Alba Silipo; Rosa Lanzetta; Michelangelo Parrilli; Antonio Molinaro is a Chemistry article available to read on EtoBox.

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## Abstract A new sample preparation method for matrix‐assisted laser desorption/ionization (MALDI) analysis of native rough‐type lipopolysaccharides (R‐type LPSs) is presented. In our MALDI mass spectra, besides the [MH]^−^ ions, abundant ions originating from the cleavage between the 3‐deoxy‐D‐__manno__‐oct‐2‐ulosonic acid (Kdo) unit and the lipid A moiety are always present, giving important pieces of information about the structure of the molecules analyzed. Remarkably, in most cases, the comparison of the MALDI mass spectra of the intact R‐type LPS with the O‐deacylated one allowed us to obtain the structure of the lipid A moiety. Copyright © 2005 John Wiley & Sons, Ltd.

Who reads New conditions for matrix-assisted laser desorption/ionization mass spectrometry of native bacterial R-type lipopolysaccharides?

Author

Luisa Sturiale; Domenico Garozzo; Alba Silipo; Rosa Lanzetta; Michelangelo Parrilli; Antonio Molinaro

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2005

Language

EN

Field

Chemistry (Physical Sciences)