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In situ thermal diagnostics of the micro-PCR system using liquid crystals by Jermim Noh; Su Whan Sung; Min Ku Jeon; Soo Ho Kim; Luke P. Lee; Seong Ihl Woo is a Engineering article available to read on EtoBox.

What is In situ thermal diagnostics of the micro-PCR system using liquid crystals about?

A simple, reliable liquid crystal (LC) thermometry of a polymerase chain reaction (PCR) chip that has a microchamber inside the chip and a film heater and a film sensor patterned on the surface is firstly introduced. The silicon-based PCR chip is made by MEMS technology. A non-linear feedback proportional-integral control scheme adjusts the power input of the Pt heater to control the surface temperature of the micro-PCR chip measured by the Pt sensor with an accuracy of less than ±0.1 • C at steady state. We use the LC thermometry to analyze the temperature distribution in the microchamber of the micro-PCR chip. The reflected colors of LC's inside the microchamber are captured through a charge-coupled device (CCD) camera, subsequently analyzed using a hue-based color analysis system. Two LC's of which individual working range is near the typical PCR temperature of 55 • C and 72 • C are calibrated in a constant temperature bath with a resolution of less than ±0.3 • C. While the average microchamber temperature near the annealing temperature is similar to the surface temperature, it is lower than the surface temperature by ca. 1 • C in the range of the extension temperature. Also, th

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Author

Jermim Noh; Su Whan Sung; Min Ku Jeon; Soo Ho Kim; Luke P. Lee; Seong Ihl Woo

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0924-4247)

Published

2005

Language

EN

Field

Engineering (Physical Sciences)